the empty pcdna3.1 vector was used as the negative control (nc)  (Thermo Fisher)

Journal: Heliyon

Article Title: CircACTR2 promotes bladder cancer progression through IKBKB-mediated NF-κB signaling pathway activation

doi: 10.1016/j.heliyon.2024.e30778

Figure Lengend Snippet: Sequences used in transfections.

Article Snippet: For IKBKB overexpression, the entire sequence of IKBKB was sub-cloned into the pcDNA3.1 vector (Invitrogen, Carlsbad, CA, USA) to generate the pcDNA3.1/IKBKB expression vector; the empty pcDNA3.1 vector was used as the negative control (NC).

Techniques: Transfection

Journal: Heliyon

Article Title: CircACTR2 promotes bladder cancer progression through IKBKB-mediated NF-κB signaling pathway activation

doi: 10.1016/j.heliyon.2024.e30778

Figure Lengend Snippet: IKBKB is involved in circACTR2-mediated BCa cell proliferation, invasion and migration. J82 cells were transfected with si-NC, si-circACTR2-1, or co-transfected with si-circACTR2-1 and pcDNA3.1/IKBKB for functional rescue assays. A-B. The proliferation ability of three groups of J82 cells was evaluated by CCK-8 assay (A) and EdU assay (B), respectively. C-D. The number of invaded or migrated cells was calculated using transwell invasion (C) and migration (D) assays after treatment with three different transfections. E. A wound healing assay was carried out in three groups of J82 cells to detect migration ability. **P < 0.01.

Article Snippet: For IKBKB overexpression, the entire sequence of IKBKB was sub-cloned into the pcDNA3.1 vector (Invitrogen, Carlsbad, CA, USA) to generate the pcDNA3.1/IKBKB expression vector; the empty pcDNA3.1 vector was used as the negative control (NC).

Techniques: Migration, Transfection, Functional Assay, CCK-8 Assay, EdU Assay, Wound Healing Assay